People coming to the UK from France and the Netherlands will be forced to quarantine for 14 days from Saturday.
Transport Secretary Grant Shapps said the measure – which also applies to people travelling from Monaco, Malta, Turks and Caicos, and Aruba – would kick in from 04:00 BST.
He added that this was necessary to keep coronavirus infections down.
France warned the UK decision would lead to “reciprocal measures” across the Channel, BBC reported.
With up to half a million UK tourists thought to be in France at present, the deadline is expected to induce a rush to ports and airports, with thousands of tourists desperate to avoid quarantine.
The Eurotunnel website is reportedly struggling to deal with the volume of inquiries.